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Immunohistochemical evaluation of uteri on day 4.5 post-coitus in SR-BI KO/ ApoeR61 h/h mice. Leptin receptor (Leptin R) ( a-c ), cyclooxygenase (COX) −2 ( d-f ), leukaemia <t>inhibitory</t> factor <t>(LIF)</t> ( g-i ) and phospho-signal transducer and activator of transcription (Stat) −3 ( j-l ) immunostaining with haematoxylin counterstaining in SR-BI KO/ ApoeR61 h/h mice with placebo ( b , e , h , k ) or probucol treatment ( c , f , i , l ). As a control, uteri from wild type mice ( a , d , g , j ) were evaluated. Scale bar = 100 µm
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Immunohistochemical evaluation of uteri on day 4.5 post-coitus in SR-BI KO/ ApoeR61 h/h mice. Leptin receptor (Leptin R) ( a-c ), cyclooxygenase (COX) −2 ( d-f ), leukaemia inhibitory factor (LIF) ( g-i ) and phospho-signal transducer and activator of transcription (Stat) −3 ( j-l ) immunostaining with haematoxylin counterstaining in SR-BI KO/ ApoeR61 h/h mice with placebo ( b , e , h , k ) or probucol treatment ( c , f , i , l ). As a control, uteri from wild type mice ( a , d , g , j ) were evaluated. Scale bar = 100 µm

Journal: Reproductive Sciences

Article Title: Female Infertility and Risk for Later-Life Cardiovascular Disease: Lessons from a Mouse Model of Human Cardiovascular Disease

doi: 10.1007/s43032-025-02026-y

Figure Lengend Snippet: Immunohistochemical evaluation of uteri on day 4.5 post-coitus in SR-BI KO/ ApoeR61 h/h mice. Leptin receptor (Leptin R) ( a-c ), cyclooxygenase (COX) −2 ( d-f ), leukaemia inhibitory factor (LIF) ( g-i ) and phospho-signal transducer and activator of transcription (Stat) −3 ( j-l ) immunostaining with haematoxylin counterstaining in SR-BI KO/ ApoeR61 h/h mice with placebo ( b , e , h , k ) or probucol treatment ( c , f , i , l ). As a control, uteri from wild type mice ( a , d , g , j ) were evaluated. Scale bar = 100 µm

Article Snippet: The 5-μm paraffin-embedded tissue sections were immunostained using monoclonal rabbit anti-mouse phosphorylated signal transducer and activator of transcription-3 (p-Stat3) antibody (Tyr705) (#9145; Cell Signaling Technology, Tokyo, Japan) at 1:400 dilution, polyclonal rabbit anti-mouse leukaemia inhibitory factor (LIF) antibody (OABF00432; Aviva Systems biology, CA, US) at 1:400 dilution, polyclonal goat anti-mouse leptin receptor antibody (AF497; R&D Systems, MN, US) at 1:200 dilution, and polyclonal rabbit anti-mouse cyclooxygenase (COX) −2 antibody (#160,126; Cayman Chemical, MI, US) at 1:500 dilution according to the manufacturers’ instructions.

Techniques: Immunohistochemical staining, Immunostaining, Control